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TIRF-EC Microscopy |
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TM1003-01 |
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Summary:
 YOUR microscope and YOUR objectives
OUR open or closed perfusion TIRF cells
Install/uninstall in less than one minute
LED, Xenon lamp and laser illuminators
Optional electrochemical control and computer-controlled fluidics
Supported Microscopes:
Nikon
Olympus
Zeiss
Leica |
Availability: Now |
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| Overview: |
TIRF Technologies offers prism- and lightguide-based TIRF Microscopy flow systems equipped with open and closed perfusion flow chambers suitable for both inverted and up-right microscopes.
The advantages of prism-and lightguide-based TIRF systems, with respect to through-objective TIRFM, include superior contrast and signal-to-noise ratio. Water immersion and dry objectives can be used with our TIRFM systems. The excitation spot of evanescent wave is 10 mm X 6 mm or larger, which allows for simulteneous TIRF imaging of multiple objects. There is no dependency on expensive specialized TIRF through-objective lenses.
In contrast to through-objective TIRFM, with which only laser excitation can be used, prism-and lightguide-based TIRFM is compatible with inexpensive LEDs, halogen, xenon, mercury arc lamps and can also be used with lasers. This allows you to excite a full spectrum of fluorophores, as opposed to the limited selection matched to lasers.
TIRF Microscopy is ideally suited to studies of surface based molecular-cellular and molecular-molecular interactions. Such studies include single molecule detection, endo- and exocytosis, membrane trafficking, protein-protein interactions, DNA hybridization, etc.
Options include: electrochemical control, temperature control, computer-controlled fluidics, chemically modified TIRF slides and reagent kits for immobilization of biomolecules. |
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| Specifications: |
| Type of data |
TIRF imaging. Kinetics of biomolecular interactions, k[on] and k[off] rate constants, affinity constants, monitoring structural changes, lateral mobility, clustering of biomolecules, determination of distances between biomolecules. FTRET, FRAP, FCS and multifluorophore experiments. The effect of electric field and dielectrophoresis studies. |
| Type of measurement |
Real-time, non-destructive measurement of fluorescence in TIRF mode by low light CCD camera. |
| Wavelength range |
Excitation: 250-700 nm. Emission range depends on the microscope optics, typically 380-800 nm. |
| TIRFM sensor chips |
Silica or glass microscopy slides 1-mm thick or cover slips 0.17 mm with unmodified or chemically modified surfaces. |
| Bioanalyte type |
Solutions of biomolecules, suspension of cells, cell culture in the open perfusion chamber. Depending on the application fluorescent reporter can be located either at the receptor molecule on the TIRF surface or at the ligand in the solution phase. |
| Liquids handling |
Optional computer controlled fluidics SmartFlowTF1005 performs automated priming, sample injection, rinsing, regeneration, and recovery of bioanalyte solution. Alternative fluiidcs or gravity flow are compatible with TM1003 products. |
| Flow cell volume |
Closed flow cell 10 - 80 µl. Open perfusion chambers - 100-500 µl. |
| Installation and Setup |
Optional |
| Training |
Training literature and over the phone support is included. Optional on-site training is available. |
| Warranty and Support |
Off-site, included for one year. On-site and extended off-site warranty and support are available for fee. |
| Optional accessories |
Custom design perfusion chambers and PDMS microfluidics are availabe as special orders. Chemically modified TIRF and TIRF-EC slides, reagent kits for immobilization of receptor molecules. Temperature control. Electrochemical control of the TIRF-EC sensor surface. LED, laser diode, Xenon arc lamp and halogen lamp illuminators. Autosampler. Computer controlled fluidics SmartFlow TF1005. |
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