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Summary:
 Transform your spectrofluorometer into a super-sensitive TIRF biosensor instrument
Install and uninstall TIRF unit in less than one minute
Employ TIRF in combination with electrochemical and/or temperature control
Supported Spectrofluorometrs:
SPEX Fluorolog and Fluoromax
PTI
ISS K2
Varian Eclipse
SLM AB-2, 4800, 8100
Shimadzu
Hitach
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Availability: Now |
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| Overview: |
TIRF Technologies supplies TIRF flow systems as factory aligned accessories, assembled on a sample compartment of the fluorometer of your choice. Our TIRF systems have been successfully used with SPEX Fluorolog and Fluoromax, ISS K2, SLM AB-2, 4800 and 8100, Shimadzu, Hitachi, Varian Eclipse and PTI as well as home-built fluorometers. TIRF is an exceptionally sensitive and informative technique. No other technique exists that can monitor fluorescence lifetime, polarization, anisotropy decay, quenching, resonance energy transfer (FRET), recovery after photobleaching (FRAP), and correlation spectroscopy (FCS) for analysis of biomolecular interactions in real-time and with sensitivity at the level of single molecule detection. TIRF accessory TA1004 can be interfaced with our computer-controlled fluidic system TF1005 that transforms your fluorometer to a computer-controlled TIRF biosensor system. Electrochemical and temperature control are available as options. Chemically clean TIRF slides and chemically modified slides with reactive amine, aldehyde, epoxy groups, biotinylited and streptavidin coated TIRF slides and reagent kits for surface immobilization of biomolecules are available as consumables.
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| Specifications: |
| Type of data |
Kinetics of biomolecular interactions, kon and koff rate constants, affinity constants, monitoring structural changes, lateral and rotational mobility, clustering of biomolecules, determination of distances between biomolecules. |
| Type of measurement |
Real-time, non-destructive, measurement of fluorescence and fluorescence polarization in TIRF mode |
| TIRF prism and TIRF sensor chips |
Silica TIRF prism. Silica or glass 1" x 3" microscopy slides supplied with chemically modified surfaces and reagent kits for immobilization of receptor molecules. |
| Bioanalyte type |
Solutions of biomolecules. Depending on the application fluorescent reporter can be located either at the receptor molecule on the TIRF surface or at the ligand in the solution phase. |
| Liquids handling |
Manual operations and external pump are needed. TA1004-01 TIRF accessory interfaced with our TIRF fluidics TF5, which provides automated priming, sample injection and rinsing. |
| Sample volume |
100-250µl |
| Flow chamber volume |
10 µl |
| Size of accessory |
Fits in the sample compartment of SPEX Fluorolog and Fluoromax, ISS K2, SLM AB-2, 4800 and 8100, Shimadzu, Hitachi, and PTI, Eclipse and other commercial fluorometers. |
| Installation and setup |
Optional |
| Training |
Optional |
| Warranty and support |
Off-site warranty included for one year. Extended on-site and off-site warranty and support available for fee. |
| PC requirements |
MS Windows XP, 2Ghz Processor, 512MB RAM, 100Mb available disk space, CD-ROM, USB2 port. |
| Optional accessories |
Chemically modified TIRF and TIRF-EC slides, reagent kits for immobilization of receptor molecules. Electrochemical control of the TIRF-EC sensor surface. Temperature control. Computer controlled TIRF Fluidics TF5. |
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