Our new name is TIRF Labs, new website: www.tirf-labs.com - TIRF Microscopy and Spectroscopy, TIRF Biosensors, TIRF Slides

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Visit our new website: www.tirf-labs.com

Our new name is TIRF Labs. Please visit our new website: www.tirf-labs.com


Total Internal Reflection Fluorescence (TIRF) employs the phenomenon of total internal reflection, which occurs at the interface between optically dense medium, such as glass, and optically less dense medium –water or aqueous solution. At large angle of incidence, the excitation beam reflects back into glass and generates at the interface with water so called evanescent wave. The evanescent wave has maximum of intensity at the surface and exponentially decays with the distance from the interface. Only molecules that are at the TIRF surface are excited and fluoresce, while molecules in the bulk of solution, at the distances larger than 100-200 nm are not excited and, respectively, do not fluoresce. (See TIRF Principles.) TIRF efficiently rejects background signal from the bulk of solution and allows for super sensitive detection - down to single molecules. In fact, it has become a method of choice for single molecule detection experiments.(SMD)

TIRF is real-time, low volume (< 0.1 μl), in situ technique, which is well-suited for analysis of biomolecular interactions and studies of the mechanisms of biomolecular events.

In contrast to traditional microarrays that detect only end-point results, TIRF measures the entire course of interactions, determines k-on and k-off kinetic rate constants, and probes their dependence on electrochemical polarization. TIRF is capable of massively parallel analysis - thousands of different assays can be microarrayed on the surface of single TIRF slide and, respectively, thousands of DNA/RNA and protein targets simultaneously detected in small sample. Additionally, exceptional surface selectivity permits TIRF to analyze scattering and turbid samples, such as whole blood, with no or minimum sample preparation.

TIRF Technologies offers broad range of TIRF and TIRF-electrochemical systems for detection and control of biomolecular interaction such as protein-protein, receptor-ligand, protein-DNA, DNA-DNA, protein-membrane, and other biomolecular interactions.

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