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TIRF microscopy, TIRF flow system for fluorometers, TIRF biosensor instruments, Fluorescence illuminators, TIRF electrochemistry, TIRF dielectrophoresis, Real-time DNA, RNA, protein, and metabolite TIRF microarrays, TIRF slides, TIRF lightguides, TIRF prisms.                Contact us: info@tirf-labs.com

TIRF Microscopy

TIRF Spectroscopy

iTIRF Molecular Diagnostics

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Fluorescence Illuminators LED/Lasers

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TIRF Microscopy  

TIRF for Fluorometers


TIRF Biosensors

TIRF Slides, TIRF Prisms

TIRF - Total Internal Reflection Fluorescence

also known as evanescent wave or evanescent field fluorescence

  TIRF Principles. TIRF employs the phenomena of total internal refection and evanescent wave to selectively excite fluorophores located at the surface and in close proximity (100-200 nm) to the surface of a glass, silica, or plastic slide. Fluorophores that are in the bulk of solution are not excited and do not fluoresce. Exceptional spatial selectivity enables TIRF with numerous advantages, including the ultimate limit of detection - single molecule. TIRF efficiently minimizes the background and allows for monitoring in real time the molecular dynamics at the level of single molecules.  More »

    TIRF Applications. TIRF spectroscopy and TIRF microscopy are powerful analytical tools for numerous life science areas, including analysis of biomolecular interactions, single molecule detection, cell biology,  real-time microarrays for molecular diagnostics, bioassay development, nanoengineering, and drug screening. In particular, TIRF is “a method uniquely suited to image the plasma membrane with its associated organelles and macromolecules in living cells. The method shows even the smallest vesicles made by cells, and can image the dynamics of single protein molecules.” [Steyer JA, Almers W. A real-time view of life within 100 nm of the plasma membrane. Nature Reviews, Molecular Cell Biology, 2001, 2(4), 268-75]. More »

   TIRF Geometries.  Prism-, objective-, and lightguide-based geometries produce the evanescent wave. Each geometry has its own set of advantages and limitations. Prism-based scheme provides the best signal-to-background ratio, but is difficult to implement with open perfusion chamber on an inverted microscope. Objective-based scheme is compatible with open perfusion chamber, but the background is larger, the intensity of evanescent wave is irreproducible, and one must use specialized objectives with NA >1.43. Lightguide-based geometry yields exceptional flexibility - can be used with dry, water-, and oil-immersion objectives, is well-suited for multicolor TIRF, but requires larger optical power to obtain equal intensity of the evanescent wave.  More »

    TIRF Products. TIRF Labs offers broad range of TIRF products: prism-, lightguide-, and objective-based TIRF microscopy  from simple add-on accessories - to sophisticated computer-controlled  turnkey installations. Our LED and laser fluorescence illuminators include cost-efficient single-color light sources and computer-controlled seven-color illuminators. For TIRF spectroscopy we supply TIRF accessory with gravity flow and digital fluidics. Our automated TIRF microarray biosensors detect multiple nucleic acid, protein, and metabolite markers in parallel and in a matter of a few minutes. Popular products also include TIRF slides, lightguides, and prisms. More »

Technologies lgTIRFM_2sidesTL.pdf TIRF Microscopy Applications Applications Applications

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Digital Fluidics SmartFlow

prism_tirfm_3.pdf prism_tirfm_3.pdf